pro bdnf  (Alomone Labs)

Journal: Frontiers in Pharmacology

Article Title: The astrocytic sigma-1 receptor constitutes in the fast antidepressant action of hypidone hydrochloride (YL-0919) in rodents

doi: 10.3389/fphar.2025.1564851

Figure Lengend Snippet: Knockdown of astrocytic sigma-1R blocked the regulatory effects of YL-0919 on mitochondrial function and BDNF expression in primary astrocytes (A) Western blotting assays for detecting the expression level of Sigma-1R in primary astrocytes; (B, C) Measurement of intracellular ATP and extracellular ATP, n = 21; (D) The mitochondrial membrane potential of primary astrocytes was measured by TMRM fluorescence; (E) mean fluorescence intensity of mitochondrial membrane potential in primary astrocytes, n = 6; (F–H) Western blot analysis of pro-BDNF and mature BDNF in primary astrocytes, n = 3; * Significant difference by two-way ANOVA, followed by Tukey’s multiple comparisons test (A–E) and one-way ANOVA, followed by Dunnett’s test (F–H) , * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The membranes were blocked with 5% skim milk for 1 h at room temperature, and incubated with the following primary antibodies against sigma-1R (1:1,000, Cell signaling technology, 61994S), pro-BDNF (1:1,000, Santa Cruz, sc-65514), BDNF (1:1,000, Abcam, ab108319) and β-actin (1:1,000, Proteintech, 60008-1-1) individually at 4°C overnight.

Techniques: Knockdown, Expressing, Western Blot, Membrane, Fluorescence